AB037. Icariside II improves human cavernous endothelial cells function by regulating miR-155/eNOS signal pathway

given a gavage of saline (0.1 mL·kg-1 ·d-1). Eight weeks later, the sexual behavior was investigated by the copulatory test, the morphological change of prostatic tissue was observed by light microscopy after HE staining, cytokines (TNF-α, IL-1β) in serum were detected by ELISA, the levels of 5-HT, 5-HT1A receptor, 5-HT2C receptor and SERT in T13-L2 and L5-S2 spinal cord tissue were detected by immunohistochemical staining and Western Blot. Results: In model group, prostatic inflammation was found in 12 rats, and not in another 4 rats. The 4 rats were not included in the statistical analysis. In normal control group, prostatic inflammation was not found. In the celecoxib treatment group, there was a small amount of interstitial infiltration of inflammatory cells in rat's prostate. In the copulatory test, compared with normal control group, mount latency (ML) and intromission latency (IL) in the model group were significantly prolonged (P<0.05); ejaculation latency (EL) in the model group was significantly shortened (P<0.05). There was no significant difference in these sexual behavior parameters between the normal control group and the celecoxib treatment group (P>0.05). Serum IL-1β and TNF-α levels in the model group were significantly higher than that in the control group (P<0.01). There was no significant difference in the levels of IL-1β and TNF-α between the normal control group and the celecoxib treatment group (P>0.05). In T13-L2 spinal cord tissue, compared with normal control group, 5-HT levels in the model group were significantly lower (P<0.05), 5-HT1A receptor levels in the model group were significantly increased (P<0.05), while the 5-HT2C receptor and SERT levels in the model group did not change significantly (P>0.05). In L5-S2 spinal cord tissues, compared with normal control group, 5-HT levels in the model group were significantly lower (P<0.05), while the 5-HT1A, 5-HT2C and SERT levels in the model group increased slightly, but the differences in 5-HT1A, 5-HT2C and SERT levels between the model group and the normal control group were statistically significant (P<0.05). There were not significant differences in 5-HT, 5-HT1A, 5-HT2C and SERT levels between the normal control group and the celecoxib treatment group (P>0.05). Conclusions: EAP shorten rat's EL by changing the levels of 5-HT and its receptors, SERT in spinal cord. Cox-2 inhibitors can prolong EL of EAP rat, the mechanisms might be that it can relieve inflammation of the prostate, reverse the effect of EAP on spinal cord 5-HT transmitter system. Cox-2 inhibitors can be used …